Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm.
Identifieur interne : 000575 ( Main/Exploration ); précédent : 000574; suivant : 000576Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm.
Auteurs : S M H. Alavi [République tchèque] ; D. Gela ; M. Rodina ; O. LinhartSource :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [ 1531-4332 ] ; 2011.
English descriptors
- KwdEn :
- Animals, Calcium (pharmacology), Calcium (physiology), Calcium Channel Blockers (pharmacology), Calcium-Calmodulin-Dependent Protein Kinases (antagonists & inhibitors), Chelating Agents (pharmacology), Egtazic Acid (pharmacology), Fish Proteins (antagonists & inhibitors), Fishes, Male, Nifedipine (pharmacology), Osmolar Concentration, Potassium (pharmacology), Potassium (physiology), Sperm Motility (drug effects), Sperm Tail (drug effects), Sperm Tail (metabolism), Sperm Tail (physiology), Sulfonamides (pharmacology).
- MESH :
- chemical , antagonists & inhibitors : Calcium-Calmodulin-Dependent Protein Kinases, Fish Proteins.
- chemical , pharmacology : Calcium, Calcium Channel Blockers, Chelating Agents, Egtazic Acid, Nifedipine, Potassium, Sulfonamides.
- chemical , physiology : Calcium, Potassium.
- drug effects : Sperm Motility, Sperm Tail.
- metabolism : Sperm Tail.
- physiology : Sperm Tail.
- Animals, Fishes, Male, Osmolar Concentration.
Abstract
The present study shows the roles of osmolality, calcium (Ca(2+))-potassium (K(+)) antagonist and Ca(2+) in sperm activation and flagellar beating of a sturgeon species, sterlet (Acipenser ruthenus). Sperm motility was activated at hypoosmolality relative to seminal plasma and suppressed at 175 mOsmol kg(-1). Sperm activation was totally suppressed by 0.35mM K(+), but Ca(2+) could fully reverse K(+) inhibitory effect at Ca(2+): K(+) ratio of 0.25. Neither EGTA (a chelator of Ca(2+) ions) nor nifedipine (a Ca(2+) channel blocker) prevented sperm activation. But, sperm motility and velocity were significantly decreased by EGTA, nifedipine and an inhibitor for Ca(2+)/calmodulin activated phosphodiesterase (w-7) that suggest role of Ca(2+) signaling after triggering sperm activation through hypoosmolality. Symmetric flagellar beating was also turned to asymmetric after activation in w-7, which is an evidence for modulation of Ca(2+)-binding proteins activity. Sturgeon sperm, similar to salmonids, is immotile in seminal plasma due to high K(+) concentrations, but the mechanism of sperm activation seems to be closer to other fish species where osmolality prohibits sperm activation in seminal plasma. In these species, hypoosmolality is the primary signal for sperm Ca(2+)-dependent signaling of axonemal beating.
DOI: 10.1016/j.cbpa.2011.05.026
PubMed: 21651987
Affiliations:
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Alavi</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemia Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodňany 389 25, Czech Republic. alavi@frov.jcu.cz</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemia Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodňany 389 25</wicri:regionArea><wicri:noRegion>Vodňany 389 25</wicri:noRegion></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D" last="Gela">D. Gela</name></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M" last="Rodina">M. Rodina</name></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21651987</idno><idno type="pmid">21651987</idno><idno type="doi">10.1016/j.cbpa.2011.05.026</idno><idno type="wicri:Area/PubMed/Corpus">000341</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000341</idno><idno type="wicri:Area/PubMed/Curation">000341</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000341</idno><idno type="wicri:Area/PubMed/Checkpoint">000341</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000341</idno><idno type="wicri:Area/Ncbi/Merge">000385</idno><idno type="wicri:Area/Ncbi/Curation">000385</idno><idno type="wicri:Area/Ncbi/Checkpoint">000385</idno><idno type="wicri:Area/Main/Merge">000584</idno><idno type="wicri:Area/Main/Curation">000575</idno><idno type="wicri:Area/Main/Exploration">000575</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Roles of osmolality, calcium - Potassium antagonist and calcium in activation and flagellar beating pattern of sturgeon sperm.</title><author><name sortKey="Alavi, S M H" sort="Alavi, S M H" uniqKey="Alavi S" first="S M H" last="Alavi">S M H. Alavi</name><affiliation wicri:level="1"><nlm:affiliation>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemia Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodňany 389 25, Czech Republic. alavi@frov.jcu.cz</nlm:affiliation><country xml:lang="fr">République tchèque</country><wicri:regionArea>University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemia Research Center of Aquaculture and Biodiversity of Hydrocenoses, Vodňany 389 25</wicri:regionArea><wicri:noRegion>Vodňany 389 25</wicri:noRegion></affiliation></author><author><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D" last="Gela">D. Gela</name></author><author><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M" last="Rodina">M. Rodina</name></author><author><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name></author></analytic><series><title level="j">Comparative biochemistry and physiology. Part A, Molecular & integrative physiology</title><idno type="eISSN">1531-4332</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Calcium (pharmacology)</term><term>Calcium (physiology)</term><term>Calcium Channel Blockers (pharmacology)</term><term>Calcium-Calmodulin-Dependent Protein Kinases (antagonists & inhibitors)</term><term>Chelating Agents (pharmacology)</term><term>Egtazic Acid (pharmacology)</term><term>Fish Proteins (antagonists & inhibitors)</term><term>Fishes</term><term>Male</term><term>Nifedipine (pharmacology)</term><term>Osmolar Concentration</term><term>Potassium (pharmacology)</term><term>Potassium (physiology)</term><term>Sperm Motility (drug effects)</term><term>Sperm Tail (drug effects)</term><term>Sperm Tail (metabolism)</term><term>Sperm Tail (physiology)</term><term>Sulfonamides (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Calcium-Calmodulin-Dependent Protein Kinases</term><term>Fish Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Calcium</term><term>Calcium Channel Blockers</term><term>Chelating Agents</term><term>Egtazic Acid</term><term>Nifedipine</term><term>Potassium</term><term>Sulfonamides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Calcium</term><term>Potassium</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Sperm Motility</term><term>Sperm Tail</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Sperm Tail</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Sperm Tail</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Fishes</term><term>Male</term><term>Osmolar Concentration</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present study shows the roles of osmolality, calcium (Ca(2+))-potassium (K(+)) antagonist and Ca(2+) in sperm activation and flagellar beating of a sturgeon species, sterlet (Acipenser ruthenus). Sperm motility was activated at hypoosmolality relative to seminal plasma and suppressed at 175 mOsmol kg(-1). Sperm activation was totally suppressed by 0.35mM K(+), but Ca(2+) could fully reverse K(+) inhibitory effect at Ca(2+): K(+) ratio of 0.25. Neither EGTA (a chelator of Ca(2+) ions) nor nifedipine (a Ca(2+) channel blocker) prevented sperm activation. But, sperm motility and velocity were significantly decreased by EGTA, nifedipine and an inhibitor for Ca(2+)/calmodulin activated phosphodiesterase (w-7) that suggest role of Ca(2+) signaling after triggering sperm activation through hypoosmolality. Symmetric flagellar beating was also turned to asymmetric after activation in w-7, which is an evidence for modulation of Ca(2+)-binding proteins activity. Sturgeon sperm, similar to salmonids, is immotile in seminal plasma due to high K(+) concentrations, but the mechanism of sperm activation seems to be closer to other fish species where osmolality prohibits sperm activation in seminal plasma. In these species, hypoosmolality is the primary signal for sperm Ca(2+)-dependent signaling of axonemal beating.</div></front></TEI><affiliations><list><country><li>République tchèque</li></country></list><tree><noCountry><name sortKey="Gela, D" sort="Gela, D" uniqKey="Gela D" first="D" last="Gela">D. Gela</name><name sortKey="Linhart, O" sort="Linhart, O" uniqKey="Linhart O" first="O" last="Linhart">O. Linhart</name><name sortKey="Rodina, M" sort="Rodina, M" uniqKey="Rodina M" first="M" last="Rodina">M. Rodina</name></noCountry><country name="République tchèque"><noRegion><name sortKey="Alavi, S M H" sort="Alavi, S M H" uniqKey="Alavi S" first="S M H" last="Alavi">S M H. Alavi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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